Small Molecule Inhibitors Protease Inhibitor Cocktails: Key Tools for Protecting Protein Integrity
Publish Time: 2024-12-05
In biological research, the production and degradation of endogenous proteins are usually maintained in a dynamic equilibrium state, ensuring that cells can maintain relatively constant protein levels under stable conditions. However, in in vitro experimental settings, this balance is often disrupted. Especially when the protein synthesis process is terminated, the protein degradation process may be significantly enhanced, seriously affecting the accuracy and reliability of experimental results. To address this challenge, scientists developed an inhibitor mixture composed of a variety of small molecules - a protease inhibitor mixture to effectively inhibit the activity of proteases and block the protein degradation process.
Protease inhibitor cocktails are particularly useful in mammalian cell lysates or tissue extracts. These samples often contain a variety of proteases that can degrade all proteins, including phosphorylated proteins, thus seriously affecting the analysis of protein composition and function in the sample. The introduction of protease inhibitor cocktails (such as AmBeed Protease Inhibitor Cocktail) has provided scientists with an effective solution. This mixture typically contains multiple broad-spectrum protease inhibitors that comprehensively inhibit protease activity in the sample, thereby protecting protein integrity.
AmBeed Protease Inhibitor Cocktail (EDTA-free, 100X in DMSO) is a high-quality protease inhibitor cocktail that contains six carefully selected broad-spectrum protease inhibitors that can effectively inhibit the activity of a variety of proteases. This mixture is not only suitable for mammalian cell lysates and tissue extracts, but can also be used in other experimental scenarios where protein integrity needs to be protected. Its 100-fold concentrated form makes it more convenient and economical to use. At the same time, its EDTA-free feature also makes it more suitable under certain experimental conditions.
In terms of preservation, AmBeed Protease Inhibitor Cocktail provides two different specifications of products (500ul and 1ml) to meet different experimental needs. For inhibitor mixtures in powder form, it is recommended to store them in a dry and sealed environment at -20°C to ensure their long-term stability and activity. For liquid inhibitor mixtures, although storage conditions at -20°C can provide a shelf life of 12 months, if conditions permit, storing them in an ultra-low temperature environment of -80°C can further extend their shelf life while maintaining a higher activity level.
In summary, small molecule inhibitor protease inhibitor cocktails are critical tools for protecting protein integrity in biological research. By inhibiting the activity of proteases, they block the protein degradation process, thereby ensuring the accuracy and reliability of experimental results. As a high-quality protease inhibitor cocktail, AmBeed Protease Inhibitor Cocktail provides scientists with an efficient, convenient and economical solution that is worthy of widespread application in related research.
