The production and degradation of endogenous proteins are mostly in a state of equilibrium, and the protein level in cells is also stable under stable conditions. However, in in vitro studies, protein synthesis is terminated and degradation is enhanced, which affects the protein yield. In order to prevent protein degradation under such conditions, people use a mixture of inhibitors composed of multiple small molecules to inhibit the action of proteases, thereby blocking protein degradation. Protease inhibitor cocktails are used in mammalian cell lysates or tissue extracts to increase protein stability. Without the protection of protease inhibitor cocktails, the proteases present in the sample will degrade all proteins including phosphorylated proteins. AmBeed Protease Inhibitor Cocktail (EDTA-free, 100X in DMSO) contains a mixture of 6 broad-spectrum protease inhibitors, which can effectively protect protein integrity. This product is available in two specifications, 500ul and 1ml, with different prices. In powder state, store in a dry and sealed place at -20℃, in liquid state, store at -20℃, with a shelf life of 12 months, in liquid state, store at -80℃, with a shelf life of 12 months.
