How to ensure the sterility and mycoplasma-free status of fetal bovine serum (superfine) through testing?
Publish Time: 2025-02-14
As a key component in cell culture, the sterility and mycoplasma-free status of fetal bovine serum (superfine) is crucial to ensure the purity of cell culture and the reliability of experimental results. In order to ensure the sterility and mycoplasma-free status of fetal bovine serum (superfine), a series of strict testing methods need to be adopted.
First of all, sterility testing is the first step to ensure the purity of fetal bovine serum (superfine). This test is usually carried out by inoculation culture. Specifically, an appropriate amount of fetal bovine serum (superfine) sample to be tested is inoculated into a suitable culture medium, and then cultured under certain temperature and humidity conditions. If there is no sign of any microbial growth in the culture medium within the prescribed culture time, the sample can be considered sterile. Common culture media include TG, GA and TSB, which can cover most common bacteria and fungi.
Secondly, mycoplasma detection is also an important link to ensure the quality of fetal bovine serum (superfine). Mycoplasma is a tiny microorganism that is difficult to detect by conventional culture methods, so a more sensitive detection method is needed. Common mycoplasma detection methods include modified Frey's liquid culture method, modified Frey's agar solid plate culture method, and PCR method. Among them, the modified Frey method determines whether there is mycoplasma contamination by observing the color change and pH change of the culture, while the PCR method detects the specific DNA sequence of mycoplasma for rapid and accurate judgment.
When performing sterility and mycoplasma detection, some operational details should also be paid attention to. For example, the experimental conditions need to be strictly controlled during the detection process to avoid any possible sources of contamination; at the same time, a highly sensitive and specific detection method needs to be used to ensure the accuracy of the test results.
In summary, the sterility and mycoplasma-free status of fetal bovine serum (superfine) can be ensured through sterility detection and mycoplasma detection. These detection methods not only help to ensure the purity of cell culture and the reliability of experimental results, but are also an important part of fetal bovine serum (superfine) quality control. Therefore, when using fetal bovine serum (superfine) for cell culture experiments, it is important to pay attention to the detection of sterility and mycoplasma-free status.
